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Title: Surface modification of ultra thin poly (e-caprolactone) films by acrylic acid grafting and collagen immobilization
Keywords: Poly (e-caprolactone), surface modification, human dermal fibroblasts, myoblasts, collagen immobilization
Issue Date: 16-Nov-2004
Citation: CHENG ZIYUAN (2004-11-16). Surface modification of ultra thin poly (e-caprolactone) films by acrylic acid grafting and collagen immobilization. ScholarBank@NUS Repository.
Abstract: Poly (e-caprolactone) (PCL) has been used as a bioresorbable polymer in numerous medical devices as well as for tissue engineering applications. PCL surface, however, is hydrophobic and cell-biomaterial interaction is not the best. We attempt for the first time to modify an ultra thin PCL surface with collagen. The PCL film was prepared using solvent casting and biaxial stretching technique developed in our laboratory. This biaxial stretching produced an ultra thin PCL 3 to 7 mm thick. The PCL film was pretreated using Argon plasma, and then UV polymerized with acrylic acid (AAc). Collagen immobilization was then carried out. The modified film surface was characterized by Fourier Transform Infrared (FT-IR) and X-ray Photoelectron Spectroscopy (XPS). Water contact angles were also measured to evaluate the hydrophilicity of the modified surface. Results showed that the hydrophilicity of the surface has improved significantly after surface modification. The water contact angle dropped largely. Atomic Force Microscopy (AFM) showed an increase in roughness of the film. Human dermal fibroblasts and myoblasts attachment and proliferation were improved remarkably on the modified surface. The films showed excellent cell attachment and proliferation rate.
Appears in Collections:Master's Theses (Open)

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