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Title: Generation of hepatocyte-like cells from mouse embryonic stem (ES) and bone marrow (BM) cells
Authors: YIN YIJUN
Keywords: hepatocytes, Embryonic Stem Cells, Bone Marrow, transplantation
Issue Date: 2-Aug-2004
Citation: YIN YIJUN (2004-08-02). Generation of hepatocyte-like cells from mouse embryonic stem (ES) and bone marrow (BM) cells. ScholarBank@NUS Repository.
Abstract: The paucity of hepatocytes available for hepatocyte replacement therapy requires consideration of alternatives such as embryonic stem (ES) cell-derived hepatic progenitors or BM cells. Here, Green fluorescent protein (GFP) was knocked into the a- fetal protein (AFP) locus of mouse ES cells to facilitate isolation of AFP+ hepatocyte-like progenitors that differentiated into hepatocyte-like cells in vivo and expressed albumin, haptoglobin and apolipoprotein-E. In addition, intrasplenic transplantation of BM cells in mouse models of hepatic deficiency can generate hepatocyte-like cells at low frequency. These cells can be amplified to a therapeutic level when given a selective advantage over host hepatocytes in a sufficiently chronic hepatic deficient microenvironment. This thesis proposes that both ES cells and BM are viable alternative cell sources in the cell replacement therapy for liver diseases.
Appears in Collections:Ph.D Theses (Open)

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