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https://scholarbank.nus.edu.sg/handle/10635/14083
DC Field | Value | |
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dc.title | Comparative genetic analysis of the transcriptional regulatory DNA of the oxytocin and vasopressin genes | |
dc.contributor.author | PATRICK GILLIGAN | |
dc.date.accessioned | 2010-04-08T10:39:41Z | |
dc.date.available | 2010-04-08T10:39:41Z | |
dc.date.issued | 2004-09-20 | |
dc.identifier.citation | PATRICK GILLIGAN (2004-09-20). Comparative genetic analysis of the transcriptional regulatory DNA of the oxytocin and vasopressin genes. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/14083 | |
dc.description.abstract | The regulation of gene transcription is central to biology, but poorly understood. I chose sequence comparisons to identify regulatory DNA of the oxytocin and vasopressin genes. I used transgenic mice to show that the regulation is conserved between the mouse genes and their Fugu orthologues, isotocin and vasotocin, but could not detect informative non-coding sequence similarity between the mouse and Fugu, or even mouse and human genes. I postulated that under neutral evolution, transcription factor binding sites would be retained within the enhancer as a whole, but might be gained and lost at positions within the enhancer. I used "comparative gel-shifts" to identify transcription factor binding sites that were indeed a??conserveda?? in this way. These results suggest that "grammar" - in at least some enhancers - is very flexible, that enhancers might be very "evolvable" compared to coding sequences, and that a conceptually similar approach to might be used in genome annotation. | |
dc.language.iso | en | |
dc.subject | oxytocin, enhancer, transcription, cis-regulatory, evolution, transcription factor binding site | |
dc.type | Thesis | |
dc.contributor.department | INSTITUTE OF MOLECULAR & CELL BIOLOGY | |
dc.contributor.supervisor | VENKATESH B | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
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01Gilligan.pdf | 4 MB | Adobe PDF | OPEN | None | View/Download | |
Fig4.4Gilligan.pdf | 280.19 kB | Adobe PDF | OPEN | None | View/Download | |
Fig4.7Gilligan.pdf | 10.14 MB | Adobe PDF | OPEN | None | View/Download | |
Fig4.9Gilligan.pdf | 1.11 MB | Adobe PDF | OPEN | None | View/Download |
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