Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14061
Title: Applications of capillary electrophoresis in the analysis of natural products
Authors: FENG HUATAO
Keywords: Natural products; Capillary electrophoresis; Alkaloids; Herbal medicines; Chip; CE-MS
Issue Date: 14-Jun-2004
Source: FENG HUATAO (2004-06-14). Applications of capillary electrophoresis in the analysis of natural products. ScholarBank@NUS Repository.
Abstract: This work focuses on the analytical applicability of capillary electrophoresis (CE) in the analysis of natural products. To obtain enough selectivity and sensitivity, various detection methods--- optical, electrochemical and hyphenating detections --- were tested and applied in this thesis. Five metals were separated and determined in 10 min by a single run with correlation coefficients greater than 0.999. Detection limits (S/N=3) of Co, Fe, Cu, Zn and Ni varied from 6 I?g/L to 30 I?g/L. Toxic alkaloids were selected and determined successfully with UV and MS detectors. The MS detector showed better sensitivity than UV detector. Nonaqueous Capillary Electrophoresis (NACE) was applied and had provided its attractive usefulness for the separation of alkaloids. The limit of detection (LOD) was enhanced by about 8-10 times. Since the miniaturization has received considerable attention in the recent development of CE, the use of microfabrication techniques to construct CE system on a chip was explored. The plate number appeared to be comparable to that achieved by other reports.
URI: http://scholarbank.nus.edu.sg/handle/10635/14061
Appears in Collections:Ph.D Theses (Open)

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