Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/14012
Title: Fabrication, characterization and degradation of PHB and PHBV microspheres for liver cell growth
Authors: CHAW SU THWIN
Keywords: PHB and PHBV, microspheres, 3D scaffold, degradation, liver cell, tissue engineering
Issue Date: 27-Jun-2004
Source: CHAW SU THWIN (2004-06-27). Fabrication, characterization and degradation of PHB and PHBV microspheres for liver cell growth. ScholarBank@NUS Repository.
Abstract: Microbial polyester PHB, PHBV(5%), PHBV(8%) and PHBV(12%) were fabricated as 2D thin films and 3D microspheres as artificial scaffolds for liver cell growth. The degradation of the microspheres increased while hydrophilicity, crystallinity, Tm and Tg decreased with increasing HV content. ISO 10993-5 standard direct contact cytotoxicity test was evaluated using mouse fibroblast cell lines, L-929, and the results indicated that PHB and PHBV were non-cytotoxic. Human hepatoma cell lines, Hep3B, were cultured in-vitro on both the PHB and PHBV microspheres and thin films. MTT results showed that the cell proliferation on the microspheres were more than 2-5 times higher than on thin films on 6 days culture. At the same culture period, EROD and ELISA assays showed that cytochrome P-450 activity and albumin secretion respectively were 1 to 2 times higher on the microspheres than on thin films.
URI: http://scholarbank.nus.edu.sg/handle/10635/14012
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