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Title: Analysis of type IX collagen in zebrafish axial development
Keywords: Extracellular matrix, floor plate, notochord, hypochord, adhesion, Nodal
Issue Date: 11-May-2004
Citation: SUDHA PUTTUR MUDUMANA (2004-05-11). Analysis of type IX collagen in zebrafish axial development. ScholarBank@NUS Repository.
Abstract: Axial structures such, as the notochord and the floor plate are important organizing centers in the developing zebrafish embryo. They derive from the organizer and continue to be formed in the tail bud during secondary gastrulation. This study describes the expression of three zebrafish Collagen type IX chains alpha(a1, a2 and a3) in these midline tissues. All three genes were expressed in the floor plate, the notochord and the underlying hypochord, but the onset of their expression and their precise distribution within these structures varied substantially. Unlike other known axial markers, colIXa2 expression is induced initially in the precursors of the medial floor plate and hypochord. Whereas, colIXa3 is expressed in the notochordal precursors and colIXa1 in the differentiated axial structures. Morpholino oligonucleotide mediated knockdown of ColIXa2 resulted in cell migration defects, loss of tissue integrity during gastrulation and disruption of the floor plate at later stages. It also affected integrity of epithelial tissues and patterning of somites and sclerotome. Lack of ColIXa2 function compromised the ability of cells to aggregate in vitro. Expression of colIXa2 is under control of Nodal signalling, indicating that colIXa2 is a downstream mediator of this signalling pathway and is involved in the integrity and coherence of floor plate.
Appears in Collections:Ph.D Theses (Open)

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