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Title: | Functional studies of adenoviral-mediated gene expression of CD157 | Authors: | NG PING PING, ANGELA | Keywords: | Adeno-X Tet-off System, CD157, overexpression, phosphorylation, focal adhesion kinase, phosphoproteome | Issue Date: | 18-Jun-2004 | Citation: | NG PING PING, ANGELA (2004-06-18). Functional studies of adenoviral-mediated gene expression of CD157. ScholarBank@NUS Repository. | Abstract: | In this work, the Adeno-X Tet-off system was established in COS-7 cells and was used for functional studies of CD157. This system was found to introduce transgene at very high efficiency. Using this system, overexpression of CD157 and mCD157 in COS-7 cells was found to mediate phosphorylation of a p130 protein. This p130 protein was found to be focal adhesion kinase (FAK) and was phosphorylated on Tyr397 and Tyr861. In addition, preliminary work was also carried out to study the differential regulation of phosphorylation in COS-7 cells over-expressing CD157 using the phosphoproteomic approach. Some differences in phosphorylation were observed. | URI: | http://scholarbank.nus.edu.sg/handle/10635/13952 |
Appears in Collections: | Master's Theses (Open) |
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