Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/139099
Title: RESPONSES TO CHANGES IN LIGHT INTENSITY AND SALINITY IN MICROALGA DUNALIELLA TERTIOLECTA
Authors: SITI RADIAH BINTE SAFIE
Keywords: Dunaliella tertiolecta, osmolyte, photo-acclimation, salt-stress, halotolerant, growth
Issue Date: 2-Aug-2017
Citation: SITI RADIAH BINTE SAFIE (2017-08-02). RESPONSES TO CHANGES IN LIGHT INTENSITY AND SALINITY IN MICROALGA DUNALIELLA TERTIOLECTA. ScholarBank@NUS Repository.
Abstract: The first objective is to identify rate-limiting steps in growth as Dunaliella tertiolecta acclimates to changing light intensity. In high light (HL) to low light (LL) transition, cell count and fatty acid synthesis was significantly higher after 24 h and acetyl-CoA carboxylase could be the rate-limiting enzyme in HL to LL response. In LL to HL transition, genes in the protein processing in ER, protein export, microtubule formation, cell cycle and nucleosome assembly could be rate-limiting in HL response. The second objective is to identify osmolytes present in D. tertiolecta and mechanisms to survive in high salinity condition. At low and intermediate salinity, the total osmotic pressure from glycerol, mannose, D-allose and internal sodium was almost equivalent to that of the medium osmotic pressure. At high salinity, cells increased unsaturated fatty acids and glycerol as well as increased gene expression of nitrate transporters and carbonic anhydrases.
URI: http://scholarbank.nus.edu.sg/handle/10635/139099
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Final thesis.pdf17.13 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

120
checked on Oct 11, 2018

Download(s)

49
checked on Oct 11, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.