Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/139083
Title: INVESTIGATION OF LACTIC ACID BACTERIAL METABOLISM FOR PROBIOTIC AND CELL FACTORY APPLICATION USING SYSTEMS BIOLOGY APPROACHES
Authors: LOKANAND KODURU
ORCID iD:   orcid.org/0000-0001-9223-8742
Keywords: Lactic acid bacteria, systems biology, genome-scale metabolic modeling, probiotics, cell factories, transcriptional regulator targeting
Issue Date: 27-Sep-2017
Citation: LOKANAND KODURU (2017-09-27). INVESTIGATION OF LACTIC ACID BACTERIAL METABOLISM FOR PROBIOTIC AND CELL FACTORY APPLICATION USING SYSTEMS BIOLOGY APPROACHES. ScholarBank@NUS Repository.
Abstract: Lactic acid bacteria (LAB) are categorized into homo-, facultative hetero- and obligate heterolactic groups, depending on the nature of fermentative metabolism they use. Here, GEMs of six different LAB, Lactococcus lactis, Lactobacillus salivarius, Lactobacillus plantarum, Lactobacillus casei, Leuconostoc mesenteroides, and Lactobacillus fermentum, two from each of the three fermentative groups were developed and validated quantitatively. Classical flux balance analysis (FBA) sufficiently captured the obligate heterofermentative behaviour and delineated their unique energy and redox metabolism. However, FBA with additional macromolecular crowding constraints was required to describe the homo- and facultative-heterofermentative behaviour. These methods along with comparative genomic analysis were then employed to investigate the probiotic capabilities of each LAB. Furthermore, a computational algorithm to identify transcriptional regulator targets for engineering LAB cell factories was developed. Overall, this study explained several characteristics distinctive to various fermentative groups of LAB and provided systems biological frameworks to evaluate their probiotic and cell factory applications.
URI: http://scholarbank.nus.edu.sg/handle/10635/139083
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