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Title: Site-directed mutagenesis of CD38 in cho tet-off cells
Authors: SHEN YIRU
Keywords: CD38 mutagenesis ADP-ribosyl cyclase calcium signaling
Issue Date: 3-May-2004
Citation: SHEN YIRU (2004-05-03). Site-directed mutagenesis of CD38 in cho tet-off cells. ScholarBank@NUS Repository.
Abstract: CD38 acts as a bifunctional ectoenzyme, synthesizing (ADP-ribosyl cyclase) and degrading (cyclic ADP-ribose (cADPR) hydrolase) cADPR, a calcium mobilizer from intracellular pool. CD38 internalization has been proposed as a mechanism by which the ectoenzyme produced intracellular cADPR, and thiol compounds have been shown to induce internalization. Here, we show that the function of ADP-ribosyl cyclase which may be important for its ligand-induced internalization of CD38. In this study, we have constructed several mutants of rat CD38 (C122K, L149V, W162G and E229G) and studied their expressions in Chinese Hamster Ovary (CHO) Tet-off cells. These results have led us to speculate the existence of ADP-ribosyl cyclase activity which may assist in controlling ligand-dependent internalization and possible down-stream production of intracellular cADPR in mediating calcium signaling.
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