Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13883
Title: Proteome analysis of hepatitis B virus-associated human hepatocellular carcinoma
Authors: LIANG MUI YEE ROSA, CYNTHIA
Keywords: Proteomics, Hepatocellular carcinoma, Two-dimensional gel electrophoresis, Mass spectrometry
Issue Date: 15-Apr-2004
Source: LIANG MUI YEE ROSA, CYNTHIA (2004-04-15). Proteome analysis of hepatitis B virus-associated human hepatocellular carcinoma. ScholarBank@NUS Repository.
Abstract: In this thesis, a proteomic approach was used to screen for differentially expressed proteins found in hepatitis B virus-associated hepatocellular carcinoma tissue with the aim of understanding the molecular mechanisms involved in hepatocarcinogenesis. Proteins extracted from paired samples of the non-tumorous and tumourous regions of both well- and poorly-differentiated liver samples infected with HBV were separated by high resolution two-dimensional gel electrophoresis (2-DE). The 2-DE profile of the well-differentiated liver sample showed 136 differentially expressed protein spots while the corresponding profile for the poorly-differentiated sample exhibited 222 differentially expressed protein spots. These proteins were subjected to MS analyses (MALDI-TOF and nESI-MS) and their identities established. The proteins were then catalogued according to their biological functions. The results of this study, with further validations, will serve as a useful resource and represent a significant first step towards the understanding of the mechanism of hepatocarcinogenesis.
URI: http://scholarbank.nus.edu.sg/handle/10635/13883
Appears in Collections:Master's Theses (Open)

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03_Material_and_methods.pdf112 kBAdobe PDF

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04_Results.pdf6.22 MBAdobe PDF

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10_References.pdf133.87 kBAdobe PDF

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