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https://scholarbank.nus.edu.sg/handle/10635/13883
Title: | Proteome analysis of hepatitis B virus-associated human hepatocellular carcinoma | Authors: | LIANG MUI YEE ROSA, CYNTHIA | Keywords: | Proteomics, Hepatocellular carcinoma, Two-dimensional gel electrophoresis, Mass spectrometry | Issue Date: | 15-Apr-2004 | Citation: | LIANG MUI YEE ROSA, CYNTHIA (2004-04-15). Proteome analysis of hepatitis B virus-associated human hepatocellular carcinoma. ScholarBank@NUS Repository. | Abstract: | In this thesis, a proteomic approach was used to screen for differentially expressed proteins found in hepatitis B virus-associated hepatocellular carcinoma tissue with the aim of understanding the molecular mechanisms involved in hepatocarcinogenesis. Proteins extracted from paired samples of the non-tumorous and tumourous regions of both well- and poorly-differentiated liver samples infected with HBV were separated by high resolution two-dimensional gel electrophoresis (2-DE). The 2-DE profile of the well-differentiated liver sample showed 136 differentially expressed protein spots while the corresponding profile for the poorly-differentiated sample exhibited 222 differentially expressed protein spots. These proteins were subjected to MS analyses (MALDI-TOF and nESI-MS) and their identities established. The proteins were then catalogued according to their biological functions. The results of this study, with further validations, will serve as a useful resource and represent a significant first step towards the understanding of the mechanism of hepatocarcinogenesis. | URI: | http://scholarbank.nus.edu.sg/handle/10635/13883 |
Appears in Collections: | Master's Theses (Open) |
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01_Title_and_content.pdf | 88.8 kB | Adobe PDF | OPEN | None | View/Download | |
02_Introduction.pdf | 87.71 kB | Adobe PDF | OPEN | None | View/Download | |
03_Material_and_methods.pdf | 112 kB | Adobe PDF | OPEN | None | View/Download | |
04_Results.pdf | 6.22 MB | Adobe PDF | OPEN | None | View/Download | |
05_Table_2.pdf | 104.23 kB | Adobe PDF | OPEN | None | View/Download | |
06_Table_3.pdf | 111.95 kB | Adobe PDF | OPEN | None | View/Download | |
07_Table_4.pdf | 88.25 kB | Adobe PDF | OPEN | None | View/Download | |
08_Table_5.pdf | 141.7 kB | Adobe PDF | OPEN | None | View/Download | |
09_Discussion.pdf | 254.23 kB | Adobe PDF | OPEN | None | View/Download | |
10_References.pdf | 133.87 kB | Adobe PDF | OPEN | None | View/Download |
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