Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/138681
Title: CHARACTERIZATION OF SURVIVAL MOTOR NEURON DYNAMICS BY FLUORESCENCE CORRELATION SPECTROSCOPY AND NEUREXIN2A FUNCTION IN A ZEBRAFISH MODEL FOR SPINAL MUSCULAR ATROPHY
Authors: ANGELA KOH PHEI SAN
Keywords: spinal muscular atrophy, survival motor neuron, neurexin2a, zebrafish, fluorescence correlation spectroscopy
Issue Date: 4-Aug-2017
Citation: ANGELA KOH PHEI SAN (2017-08-04). CHARACTERIZATION OF SURVIVAL MOTOR NEURON DYNAMICS BY FLUORESCENCE CORRELATION SPECTROSCOPY AND NEUREXIN2A FUNCTION IN A ZEBRAFISH MODEL FOR SPINAL MUSCULAR ATROPHY. ScholarBank@NUS Repository.
Abstract: Spinal Muscular Atrophy (SMA) is a progressive neurodegenerative disease, affecting lower motor neurons and caused by mutations in the Survival Motor Neuron (SMN) gene. I used fluorescence correlation spectroscopy (FCS) to study the dynamics of SMN protein in zebrafish embryos <I>in vivo</I>. In motor neurons, SMN was found to exist in a freely diffusing particle and a complex-bound form. Previously, our lab identified <i>neurexin2a</i> (<i>nrxn2a</i>) as novel target for SMA. To study the long-term effects of a <i>nrxn2a</i> deficiency, I generated <i>nrxn2a</i> knockout zebrafish and observed defective motor axons in maternal-zygotic <i>nrxn2a</i> mutants consistent with the phenotype described in SMN morphants. Adult mutants also displayed symptoms of muscular atrophy. Interestingly, adult zygotic mutants showed increased anxiety and lower aggressiveness in behavioral tests. My findings suggest that <i>nrxn2a</i> is important for synapse formation at the neuromuscular junction. The generated <i>nrxn2a</i> mutant is a suitable anxiety model for future drug screening.
URI: http://scholarbank.nus.edu.sg/handle/10635/138681
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