Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13844
Title: Expression and function of glial cell-line derived neurotrophic factor family receptor alpha 1 alternatively spliced isoforms
Authors: PENG ZHONGNI
Keywords: Glial cell-line derived neurotrophic factor; GDNF family receptor N1; Neurturin; Ret receptor tyrosine kinase; Parkinsos disease; Real time CR
Issue Date: 12-Apr-2004
Source: PENG ZHONGNI (2004-04-12). Expression and function of glial cell-line derived neurotrophic factor family receptor alpha 1 alternatively spliced isoforms. ScholarBank@NUS Repository.
Abstract: Glial cell-line derived neurotrophic factor family (GDNF) is a potent neurotrophic factor which promotes the development and survival of many neurons. GDNF binds specifically to GFRI?1 (GDNF family receptor), which mediates the activation of Ret protein-tyrosine kinase. GFRI?1a and GFRI?1b are two spliced isoforms of GFRI?1 with GFRI?1a containing an extra 15 nucleotide compared to GFRI?1b. Currently, the functional and physiological significance of the two spliced isoforms remains to be elucidated. Furthermore, the distribution and expression levels of these two isoforms are unknown. In this study, the mechanisms involved in signaling pathway, functional roles involved in cell morphological differentiation and proliferation of GFRI?1a and GFRI?1b were investigated. Transcriptional expression levels of GFRI?1a and GFRI?1b in human tissues were measured by real-time PCR.Neurturin stimulated cells expressing GFRI?1a and GFRI?1b resulted in the activation of MAPK (Erk1/2). Stimulation of GFRI?1a but not GFRI?1b resulted in the differentiation of transfected cells. The rates of proliferation were similar in the transfected cells. Real time PCR revealed similar transcriptional expression levels of GFRI?1a and GFRI?1b in human brain, but the expression of GFRI?1b was found to be significantly higher than GFRI?1a in the peripheral tissues. In conclusion, the two spliced isoforms, GFRI?1a and GFRI?1b are differentially distributed in the human tissues and showed remarkable differences in their functions.
URI: http://scholarbank.nus.edu.sg/handle/10635/13844
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