Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/138186
Title: SUMOYLATION IN REPRESSING RETROVIRUSES IN MOUSE PLURIPOTENT STEM CELLS
Authors: YU TAO
Keywords: sumoylation, retrovirus, endogenous retrovirus, pluripotency, epigenetic silencing, de-sumoylation
Issue Date: 20-Jul-2017
Source: YU TAO (2017-07-20). SUMOYLATION IN REPRESSING RETROVIRUSES IN MOUSE PLURIPOTENT STEM CELLS. ScholarBank@NUS Repository.
Abstract: Retroviral silencing has been considered as a unique feature of pluripotent stem cells. Previous studies identified a canonical KRAB-ZFP-TRIM28 axis in the regulation of retroviruses. Through a genome-wide siRNA screen, we identified novel regulators of retroviral silencing. Biochemical analysis reveals that a specific modifier, SUMO2, targets TRIM28 to orchestrate the retroviral silencing events. The SUMO2 modification is important for the recruitment of TRIM28 on retroviral sequences. Moreover, a SUMO-specific protease, SENP6, maintains a proper length of poly-sumoylation on TRIM28 and prevents it from further ubiquitination and degradation. These results suggest a fine-tuned balance of sumoylation and de-sumoylation in the regulation of retroviruses. Our finding provides an additional layer that post-translational modifications could mediate retroviral silencing by facilitating protein-protein interactions or adjusting the stability of key regulators. It also inspires us to explore other types of ZFPs, since KRAB domain is no longer necessary if the ZFPs possess multiple SUMO interaction motifs.
URI: http://scholarbank.nus.edu.sg/handle/10635/138186
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