Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.antiviral.2017.06.012
Title: A cross-clade H5N1 influenza A virus neutralizing monoclonal antibody binds to a novel epitope within the vestigial esterase domain of hemagglutinin
Authors: Paul, Subha Sankar
MOK CHEE KENG 
Mak, Tze-Minn
Ng Oi Wing 
Aboagye, James Odame
Wohlbold, Teddy John
Krammer, Florian
TAN YEE JOO 
Keywords: Epitope mapping
Hemagglutinin
Influenza H5N1
Neutralizing monoclonal antibody
Issue Date: 1-Aug-2017
Publisher: Elsevier B.V.
Citation: Paul, Subha Sankar, MOK CHEE KENG, Mak, Tze-Minn, Ng Oi Wing, Aboagye, James Odame, Wohlbold, Teddy John, Krammer, Florian, TAN YEE JOO (2017-08-01). A cross-clade H5N1 influenza A virus neutralizing monoclonal antibody binds to a novel epitope within the vestigial esterase domain of hemagglutinin. Antiviral Research 144 : 299-310. ScholarBank@NUS Repository. https://doi.org/10.1016/j.antiviral.2017.06.012
Abstract: The sporadic outbreaks of highly pathogenic H5N1 avian influenza virus have raised public health concerns. Monoclonal antibodies (MAbs) against hemagglutinin (HA) have been increasingly used successfully for therapeutic purposes. Previously, MAb 9F4, generated against clade 1 H5N1 HA, was observed to have cross-clade neutralizing efficacy and inhibited viral entry by preventing the pH-mediated conformational change of HA. Furthermore, mouse-human chimeric MAb 9F4 was found to retain high degrees of neutralizing activity. In this study, through escape mutant generation and in-silico prediction, it was revealed that MAb 9F4 binds to a novel epitope in the vestigial esterase sub-domain of HA comprising at least three non-continuous amino acid residues, arginine (R) at position 62, tryptophan (W) at position 69 and phenylalanine (F) at position 79, which interacted with MAb 9F4 in a conformation-dependent manner. Binding and neutralization studies suggested that R62 is the critical residue for MAb 9F4 binding whereas W69 and F79 seem to cooperate with R62 to stabilize the epitope. Mutation of either R62 or W69 did not affect replicative fitness of the virus in vitro. Interestingly, MAb 9F4 retained neutralizing efficacy against a clade 2.3.2.1a H5N1 virus consisting of an arginine to lysine substitution at position 62 in HA.
Source Title: Antiviral Research
URI: http://scholarbank.nus.edu.sg/handle/10635/137842
ISSN: 01663542
DOI: 10.1016/j.antiviral.2017.06.012
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