Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/137741
Title: PROBIOTIC-BASED ANTI-BIOFILM THERAPY FOR BACTERIAL-FUNGAL MIXED SPECIES BIOFILMS
Authors: NEHA SRIVASTAVA
ORCID iD:   orcid.org/0000-0001-6877-0771
Keywords: Streptococcus mutans, Candida albicans, Lactobacillus plantarum supernatant, glucosyltransferase, dental caries, probiotics
Issue Date: 18-Aug-2017
Source: NEHA SRIVASTAVA (2017-08-18). PROBIOTIC-BASED ANTI-BIOFILM THERAPY FOR BACTERIAL-FUNGAL MIXED SPECIES BIOFILMS. ScholarBank@NUS Repository.
Abstract: Streptococcus mutans is the principal biofilm forming oral pathogen associated with dental caries. Studies have shown that Candida albicans, a commensal oral fungi forms pathogenic mixed-species biofilms with S. mutans. Conventional antimicrobial treatment of bacterial and fungal infection is challenging due to antimicrobial resistance of biofilm mode of growth. Although studies have demonstrated the ability of probiotics to inhibit S. mutans and C. albicans single-species biofilms, its effect on mixed-species biofilms remains unexplored. This study evaluated the efficacy of secretory components of Lactobacillus plantarum against S. mutans and C. albicans single and mixed-species biofilms. XTT reduction assay, crystal violet assay, and colony forming units counting showed that L. plantarum supernatant inhibited S. mutans and C. albicans single and mixed-species biofilms. Pre-formed mixed-species biofilms were also successfully reduced. Confocal microscopy showed poorly developed biofilm architecture in probiotic treated biofilms. Expression of S. mutans genes associated with glucosyltransferase activity and C. albicans hyphal specific genes (HWP1, ALS1 and ALS3) were down-regulated by probiotic. Altogether, the data demonstrated the capacity of L. plantarum supernatant to inhibit the S. mutans and C. albicans mixed-species biofilms. A new insight is provided on the potential of probiotic-based strategies to prevent bacterial-fungal mixed-species biofilms in dental plaque.
URI: http://scholarbank.nus.edu.sg/handle/10635/137741
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
SrivastavaNeha.pdf2.75 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

29
checked on Jan 14, 2018

Download(s)

13
checked on Jan 14, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.