Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/137642
Title: DISSECTING THE FLORAL SCENT BIOSYNTHETIC PATHWAYS IN AROMATIC PLANTS USING METABOLOMIC AND TRANSCRIPTOMIC APPROACHES
Authors: SAVITHA DHANDAPANI
ORCID iD:   orcid.org/0000-0002-9177-6546
Keywords: Metabolome, transcriptome, floral volatile organic compounds, biosynthetic pathways, champak, Plumeria
Issue Date: 2-Aug-2017
Citation: SAVITHA DHANDAPANI (2017-08-02). DISSECTING THE FLORAL SCENT BIOSYNTHETIC PATHWAYS IN AROMATIC PLANTS USING METABOLOMIC AND TRANSCRIPTOMIC APPROACHES. ScholarBank@NUS Repository.
Abstract: As floral scent is an important trait in determining the economic importance of plants, considerable efforts have been made to decipher the pathways behind the biosynthesis of volatile organic compounds (VOCs). Magnolia champaca and Plumeria rubra are popular garden trees and their aromatic flowers are widely used in flavors and fragrances industry. Despite their popularity and economic importance, little is known about the genes/pathways responsible for their floral scent production. Here, we combined metabolomics, transcriptomics, in vitro and in vivo enzyme assays to discover the active VOC biosynthesis pathways in M. champaca and P. rubra flowers. Our approach identified (R)-linalool synthase and cytochrome P450 CYP79D73 that are essential for the production of major VOCs of M. champaca and P. rubra flowers, respectively. As first reports on floral transcriptomes of M. champaca and P. rubra, these datasets facilitate better understanding of ecological functions and sustainable production of their floral VOCs.
URI: http://scholarbank.nus.edu.sg/handle/10635/137642
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