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Title: The role of ChvG in regulating homologous recombination in Agrobacterium
Authors: WANG LONG
Keywords: Agrobacterium tumefaciens; Homologous recombination; ChvG; katA; vir; recombination
Issue Date: 10-Feb-2004
Citation: WANG LONG (2004-02-10). The role of ChvG in regulating homologous recombination in Agrobacterium. ScholarBank@NUS Repository.
Abstract: This study is mainly to determine the role of a membrane-bound sensor protein ChvG in regulating homologous recombination in Agrobacterium tumefaciens. Seven A. tumefaciens mutants, including virB-, katA- and aopB-, which resulted from mini-Tn5 transposon insertions, were recombined with a chvG- mutant A6340 and a chvG+ strain A6010, separately. The results of Southern analysis showed that some unexpected event(s) had occurred during homologous recombination in chvG- recombinant strains compared with chvG+ recombinant strains. The results of DNA sequencing showed that all the recombinations in the chvG- strains occurred at the same site, thata??s katA gene. The difference of transformation efficiency was also compared between the chvG+ strain A6010 and chvG- strain A6340. The results showed that the recombination efficiency of chvG- strain A6340 was much lower than that of chvG+ strain A6010. Our data indicate that ChvG plays an important role in regulating the homologous recombination events in A. tumefaciens.
Appears in Collections:Master's Theses (Open)

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