Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/136756
Title: THE EFFECTS OF HYDROGEN SULFIDE (H2S) ON THE RELEASE OF INFLAMMATORY MEDIATORS FROM MOUSE MACROPHAGES
Authors: HUANG WEIHAO CALEB
Keywords: Hydrogen Sulfide (H2S), Inflammation, Cytokines, Macrophages
Issue Date: 11-Sep-2017
Citation: HUANG WEIHAO CALEB (2017-09-11). THE EFFECTS OF HYDROGEN SULFIDE (H2S) ON THE RELEASE OF INFLAMMATORY MEDIATORS FROM MOUSE MACROPHAGES. ScholarBank@NUS Repository.
Abstract: Hydrogen sulfide (H2S) is a gasotransmitter produced endogenously by enzymes CSE, CBS and 3-MST, or exogenously by H2S-donor compounds. H2S modulates the inflammatory response though no clear consensus exists regarding its pro- or anti-inflammatory effects. In this study, exogenous H2S from NaHS and a novel slow H2S-releaser, FW1256, reduced inflammatory mediators secreted from LPS-stimulated mouse macrophages. FW1256 inhibited NFκB activation in these cells and reduced pro-inflammatory cytokine release in the LPS model of sepsis in the mouse. Using CRISPR-mediated gene editing, absence of CSE decreased iNOS and COX-2 expression in LPS-stimulated RAW264.7 cells, suggesting a differential role between exogenous and endogenous H2S. H2S from both NaHS and FW1256 also inhibited NLRP3 inflammasome activation in primary mouse macrophages by disrupting protein-protein binding in the NLRP3 inflammasome complex, preserving mitochondrial integrity and reducing mitochondrial ROS production in these cells. Altogether, exogenous H2S exerts anti-inflammatory effects in mouse macrophages via multiple molecular mechanisms.
URI: http://scholarbank.nus.edu.sg/handle/10635/136756
Appears in Collections:Ph.D Theses (Open)

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