Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/136741
Title: RNA-SEQ OF TURNIP CRINKLE VIRUS INFECTION AND INTERACTION OF TURNIP CRINKLE VIRUS COAT PROTEIN AND ARABIDOPSIS SULFITE OXIDASE
Authors: WU CHAO
Keywords: RNA-SEQ, sRNA-SEQ, PLANT ANTI-VIRAL DEFENSE, TURNIP CRINKLE VIRUS, TURNIP CRINKLE VIRUS COAT PROTEIN, ARABIDOPSIS SULFITE OXIDASE
Issue Date: 16-Jan-2017
Source: WU CHAO (2017-01-16). RNA-SEQ OF TURNIP CRINKLE VIRUS INFECTION AND INTERACTION OF TURNIP CRINKLE VIRUS COAT PROTEIN AND ARABIDOPSIS SULFITE OXIDASE. ScholarBank@NUS Repository.
Abstract: RNA-Seq and sRNA-Seq were performed using A. thaliana WT and dcl1-9 mutant line. A total of 5,204 DEGs were identified in TCV-infected WT plants, while only 595 DEGs were obtained in the infected dcl1-9 mutant plants. These results suggested DCL1 plays a significant role in the activation of the host anti-TCV response. The dwarf and delayed-flowering 1 (DDF1) gene was selected for further study. Results indicated positive correlation of DDF1 gene expression and TCV accumulation in infected Arabidopsis plants. Sulfite oxidase (SO) was showed to bind with TCV coat protein (CP) and triggers sulfur enhanced defense (SED). TCV CP Trp274 was identified as the key residue for the binding. The TCV (W274A) virus renders complete loss of infectivity. AtSO competes with AGO1 to bind to TCV CP through Trp274. These novel findings suggested AtSO is a host anti-viral factor that interfering the viral silencing suppressor (VSR) functions of TCV CP.
URI: http://scholarbank.nus.edu.sg/handle/10635/136741
Appears in Collections:Ph.D Theses (Restricted)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
WUCHAO Thesis final - 15 Aug 2017.pdf5.25 MBAdobe PDF

RESTRICTED

NoneLog In

Page view(s)

16
checked on Jan 14, 2018

Download(s)

12
checked on Jan 14, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.