Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/136500
Title: MOLECULAR MECHANISMS AND BIOLOGY OF TRANSGLUTAMINASE-2 IN CORNEAL EPITHELIUM
Authors: EVELYN PNG YEN SOO
Keywords: transglutaminase-2, corneal epithelium, wound healing, paxillin, hyperosmolarity, focal adhesion
Issue Date: 14-Aug-2015
Source: EVELYN PNG YEN SOO (2015-08-14). MOLECULAR MECHANISMS AND BIOLOGY OF TRANSGLUTAMINASE-2 IN CORNEAL EPITHELIUM. ScholarBank@NUS Repository.
Abstract: Transglutaminase(TG)-2 is a ubiquitous protein known to affect skin wound healing but has not been evaluated on cornea surface. This work aims to evaluate TG-2’s functions in mitochondrial mediated apoptosis, wound closure, protein interactions and trafficking using human corneal epithelial cells. Hyperosmolar-stimulation in corneal epithelial apoptosis via caspase-dependent mitochondrial dysfunction is partially dependent on TG-2, as hyperosmolar-stimulation increased mitochondrial potential; TG-2 overexpression increased mitochondrial depolarization and caspase activities. Novel centricollation technology successfully identified several TG-2 binding proteins with known wound healing functions and evaluated their cellular distributions. TG-2 deficiency decreased the expression levels of specific organelles markers and some binding proteins. In corneal wound closure, TG-2 deficiency showed reduced adhesion, migration and phosphorylation at S178 paxillin. JNK1 may be the possible kinase to phosphorylate paxillin through unknown kinase in TG-2 dependent manner. In conclusion, TG-2 is important in the modulation of corneal epithelial cell stress, protein trafficking and wound closure.
URI: http://scholarbank.nus.edu.sg/handle/10635/136500
Appears in Collections:Ph.D Theses (Open)

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