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https://scholarbank.nus.edu.sg/handle/10635/13515
DC Field | Value | |
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dc.title | Intrathecal delivery of transgene to promote nerve regeneration | |
dc.contributor.author | SHI LEI | |
dc.date.accessioned | 2010-04-08T10:33:47Z | |
dc.date.available | 2010-04-08T10:33:47Z | |
dc.date.issued | 2004-01-26 | |
dc.identifier.citation | SHI LEI (2004-01-26). Intrathecal delivery of transgene to promote nerve regeneration. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/13515 | |
dc.description.abstract | The feasibility of gene delivery into the rat lumber subarachnoid space was evaluated following repeated intrathecal injection of PEI (polyethylenimine)-DNA complexes. A single injection with PEI/DNA complexes could lead to higher transgene expression than naked DNA. The initial high transgene expression level could persist for around 5 days. In parallel, effects of PEGylation extent upon CNS gene transfer were investigated. In repeated administration, a 70% attenuation of gene expression was observed at a 2-week interval. The attenuation was attributed to apoptotic cell death triggered by PEI-DNA complexes rather than each component alone. Meanwhile, polyethylene glycol-grafted PEI mediated transgene expression without attenuation after repeated dosing was administrated, even in those rats injected three times, with a 2-week interval between two continuous injections. Repeated administration of DNA complexed with PEG-grafted PEI through this less invasive means may prolong the transgene expression when needed, providing a potent promise for the treatment of spinal cord disorders. | |
dc.language.iso | en | |
dc.subject | Gene Transfer; Polyethylenimine; PEGylation; Spinal Cord; Repeated Administration | |
dc.type | Thesis | |
dc.contributor.department | ANATOMY | |
dc.contributor.supervisor | WANG SHU | |
dc.description.degree | Master's | |
dc.description.degreeconferred | MASTER OF SCIENCE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Master's Theses (Open) |
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Master_thesis_by_Shi_Lei_on_Jan_25_2004.pdf | 910.69 kB | Adobe PDF | OPEN | None | View/Download |
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