Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13510
Title: Characterization of organic matrix in lased enamel
Authors: VEERAPPAN GIRIJA
Keywords: CO2 laser effect, caries prevention, demineralisation, confocal microscopy, polarized light microscopy, organic matrix
Issue Date: 27-Oct-2003
Source: VEERAPPAN GIRIJA (2003-10-27). Characterization of organic matrix in lased enamel. ScholarBank@NUS Repository.
Abstract: The aim of the study is to localize the organic matrix in partially EDTA-decalcified human enamel, labelled with fluorescent dye coupled with confocal microscopy, to investigate the laser-induced morphological changes in thin enamel sections by polarized light microscopy (PLM), confocal laser scanning microscopy (CLSM), and environmental scanning electron microscopy (ESEM), and to evaluate the potential inhibitory effects of CO2 laser on enamel demineralisation. Thirty enamel sections (of 120-130 A?m, in thickness) were irradiated. The effects of three different irradiation conditions were investigated under PLM, CLSM, and ESEM. The results suggest that the type of laser-induced morphological changes depends on the energy density used (range 4.4 - 63.65 J/cm2). Confocal imaging of sections irradiated at 4.4 J/cm2 revealed a distinct appearance of the lipid stain in the centre of lased spots suggesting, coating of organic matrix on the surface of enamel prisms and in inter-prismatic spaces, whereas high energy densities revealed a heterogeneous staining pattern in the laser treated areas. Laser treated sections submitted to the pH cycling model for a period of 2 days showed inhibition of demineralization in the bluish-gray discoloured regions. These findings indicated that laser-induced protection of enamel demineralization might have been caused by morphological changes in the organic matrix of lased enamel.
URI: http://scholarbank.nus.edu.sg/handle/10635/13510
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