Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13509
Title: Analytical applications of self-assembled biomolecules
Authors: FANG AIPING
Keywords: ITO, immobilization, biomolecules, biosensor, chips
Issue Date: 27-Aug-2003
Source: FANG AIPING (2003-08-27). Analytical applications of self-assembled biomolecules. ScholarBank@NUS Repository.
Abstract: It is of great significance to stably immobilize molecules of biological interest onto surfaces based on simple and reproducible chemistries for various applications in life sciences, such as biosensor technology, large-scale genomic and functional genomic analyses. <br>Here we exploit and develop a novel surface, indium-tin oxide, for reliable and reproducible one-step covalent immobilization of biomolecules for its potential applications. The direct one-step covalent immobilization of small biomolecules and even proteins are first demonstrated on this surface. Surface analytical techniques are applied to elucidate the interfacial phenomena. The formation of covalent bonds involving the carboxyl, thiol and amine functionalities on the outer shell of proteins greatly stabilizes the biomolecules on the surface. We also show a novel approach for high-density protein arrays on ITO surface through the soft-lithographic techniques. Successful and promising applications of this covalent attachment scheme are then demonstrated in biosensor technology, development of DNA and protein chips. <br><br><br><br><br><br><br><br><br><br><br><br><br><br>
URI: http://scholarbank.nus.edu.sg/handle/10635/13509
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