Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/134992
Title: MICROFLUIDIC BIOCHIP FOR PERFUSION CULTURE AND TESTING OF HEPATOCYTES
Authors: FANG YU
Keywords: Microfluidic, chip, hepatocyte, perfusion, culture, drug
Issue Date: 18-Nov-2016
Citation: FANG YU (2016-11-18). MICROFLUIDIC BIOCHIP FOR PERFUSION CULTURE AND TESTING OF HEPATOCYTES. ScholarBank@NUS Repository.
Abstract: In light of the concerns to replace or reduce animal testing with in vitro testing, cell and tissue models are investigated. In advanced cell models, it is essential to control the microenvironment, this can be achieved with microfluidic technologies in ways traditional cell culture models fail. Biomicrofluidic technology has many benefits when used to create cell and tissue engineering models. In microfluidic devices, constant laminar flows enables transport of nutrients and wastes, high surface to volume ratio allows physiologically relevant niche environment to be designed. In this thesis, we developed highly integrated and compact microfluidic chips for long-term cell culture and drug testing of hepatocyte spheroids; simple-to-use on-chip metabolic imaging assay for toxicity testing; dielectrophoretic method for cell trapping and patterning; and co-culture model for nutraceutical efficacy studies.
URI: http://scholarbank.nus.edu.sg/handle/10635/134992
Appears in Collections:Ph.D Theses (Open)

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