Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/134687
Title: STUDY THE ROLE OF MICRO-NICHES IN EPITHELIAL APLICAL-BASAL POLARISATION
Authors: WENG ZHE
Keywords: POLARITY, EPITHELIAL, MICRO-NICHE, ZONULA ADHERENS, MICRO-WELL, PATTERNING
Issue Date: 19-Aug-2016
Source: WENG ZHE (2016-08-19). STUDY THE ROLE OF MICRO-NICHES IN EPITHELIAL APLICAL-BASAL POLARISATION. ScholarBank@NUS Repository.
Abstract: Epithelia apical-basal polarisation is important for the formation of a selective barrier. This thesis developed a novel micro-well based mini-organ approach to decouple the chemical and physical parameters, to control the 3-D micro-niches, and quantitatively assessed the localization of key polarity markers. This thesis work has reported that at single cell level, lateral input of cell-cell adhesion cue in a 3-D micro-niche is crucial for the formation of apically localized “zonula adherens” belt structure identified by an actin belt, together with PAR-3, N-WASP, ZO-1 and Claudin-3, but not supported by cell-matrix adhesion cues alone. 2-D input of cell-cell adhesion cues also induced an edge localized E-cadherin and actin belt. Taken together, this thesis demonstrates that cell-cell adhesion cues in 3-D and 2-D micro-niches are sufficient to develop “zonula adherens” like belt formation at single cell level, and creates a novel tool to quantitatively imaging and analysis of key polarity markers.
URI: http://scholarbank.nus.edu.sg/handle/10635/134687
Appears in Collections:Ph.D Theses (Restricted)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
WENG ZHE_PhD Thesis_A0089334X_Final.pdf36.59 MBAdobe PDF

RESTRICTED

NoneLog In

Page view(s)

26
checked on Jan 13, 2018

Download(s)

12
checked on Jan 13, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.