Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/134418
Title: DECONSTRUCTING CELL MOTILITY IN FIBROUS ENVIRONMENT
Authors: CHARLOTTE GUETTA
Keywords: migration, 3D, fibrous, environment, force
Issue Date: 20-Oct-2016
Source: CHARLOTTE GUETTA (2016-10-20). DECONSTRUCTING CELL MOTILITY IN FIBROUS ENVIRONMENT. ScholarBank@NUS Repository.
Abstract: In vivo, cells migrate on complex 3D fibrous matrices, which has made investigation of the key molecular and physical mechanisms that drive cell migration difficult. Using reductionist approaches to recreate in vitro a simplified fibrous environment, we investigate the molecular and mechanical properties of cell migrating along suspended fibronectin-coated nanofibers, First, we report that single cell migration under such conditions requires the formation and propagation of dynamic actin based fin-like protrusions. These protrusions propagate up to hundred micrometers from the cell body, extending the leading edge and promoting highly persistent directional movement. The waves originate from one major adhesion site at leading end of the cell body, which is linked through actomyosin contractility to another site at the back of the cell, allowing force generation, matrix deformation and cell translocation. Then, we develop a novel system associating nanofibers and micropillars in order to probe the forces generated by cells.
URI: http://scholarbank.nus.edu.sg/handle/10635/134418
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