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https://scholarbank.nus.edu.sg/handle/10635/134391
DC Field | Value | |
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dc.title | ENGINEERING THERAPEUTIC ANTIBODY CANDIDATES FOR DENGUE VIRUS | |
dc.contributor.author | LIM SHE YAH | |
dc.date.accessioned | 2016-12-30T18:00:13Z | |
dc.date.available | 2016-12-30T18:00:13Z | |
dc.date.issued | 2016-08-05 | |
dc.identifier.citation | LIM SHE YAH (2016-08-05). ENGINEERING THERAPEUTIC ANTIBODY CANDIDATES FOR DENGUE VIRUS. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/134391 | |
dc.description.abstract | Dengue is currently the most important mosquito-borne viral disease affecting the population worldwide. Yet, there are no effective therapeutics available. Recently, our laboratory has characterized a potently neutralizing antibody against dengue virus serotype 1 (DENV-1) known as 14c10. For the comprehensive pharmacokinetics and off-target binding analysis of 14c10 as part of its clinical development with GlaxoSmithKline, an anti-idiotypic antibody highly specific for 14c10 was isolated. As part of our investigation, we proposed that 14c10-like antibodies might be produced in the natural immune response against DENV-1. We also showed the feasibility of engineering bispecific antibodies (monovalent function on each arm) against viruses, with serotype-specific antibodies against DENV-1 and DENV-2. In the pursuit of a rapid and efficient method of antibody discovery, we used a novel B cell activation approach coupled with next generation sequencing for the isolation of an anti-DENV-2 based on neutralization in less than two months. | |
dc.language.iso | en | |
dc.subject | Antibody, Dengue | |
dc.type | Thesis | |
dc.contributor.department | DEAN'S OFFICE (NGS FOR INTGR SCI & ENGG) | |
dc.contributor.supervisor | PAUL A MACARY | |
dc.contributor.supervisor | BRENDON JOHN HANSON | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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LimSY.pdf | 3.69 MB | Adobe PDF | OPEN | None | View/Download |
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