Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13414
Title: Nanofabrication, architecture control, and crosslinking of collagen scaffolds and the potential in corneal tissue engineering application
Authors: ZHONG SHAOPING
Keywords: Electrospinning, collagen, cornea, tissue engineering, cell culture and nanofibers.
Issue Date: 19-Jul-2007
Source: ZHONG SHAOPING (2007-07-19). Nanofabrication, architecture control, and crosslinking of collagen scaffolds and the potential in corneal tissue engineering application. ScholarBank@NUS Repository.
Abstract: Electrospinning technique was adapted to create collagen nanofibrous scaffolds using both the reported solvent of 1,1,1,3,3,3 hexafluoro-2-propanol (HFP) and one safer solvent of acetic acid (HAc). A variety of crosslinking methods were performed to increase the biostability and mechanical strength of collagen nanofibers. Nanofibrous GAG-collagen scaffold was constructed to closely mimic native extracellular matrix (ECM), and the incorporation of GAG was found to enhance cell growth. The aligned collagen scaffold was also fabricated by one controllable electrospinning with a rotational wheel collector. This scaffold exhibited a distinct fiber alignment compared with the random fibrous one as control using a static plate collector. The elongated proliferation pattern of the cells growing on the aligned scaffold coincided with the cell morphology found in many native tissues. This study suggests the topography of the ECM may affect cellular behavior, and controlling this environment is essential in the design of scaffolds for tissue engineering
URI: http://scholarbank.nus.edu.sg/handle/10635/13414
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