Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/134126
Title: The CBP2 gene from Saccharomyces douglasii is a functional homologue of the Saccharomyces cerevisiae gene and is essential for respiratory growth in the presence of a wild-type (intron-containing) mitochondrial genome
Authors: Li, G.-Y.
Tian, G.-L. 
Slonimski, P.P.
Herbert, C.J.
Keywords: CBP2
Mitochondria
Pre-mRNA processing
Saccharomyces cerevisiae
Saccharomyces douglasii
Issue Date: 1996
Source: Li, G.-Y., Tian, G.-L., Slonimski, P.P., Herbert, C.J. (1996). The CBP2 gene from Saccharomyces douglasii is a functional homologue of the Saccharomyces cerevisiae gene and is essential for respiratory growth in the presence of a wild-type (intron-containing) mitochondrial genome. Molecular and General Genetics 250 (3) : 316-322. ScholarBank@NUS Repository.
Abstract: In Saccharomyces cerevisiae the only known role of the CBP2 gene is the excision of the fifth intron of the mitochondrial cyt b gene (bI5). We have cloned the CBP2 gene from Saccharomyces douglasii (a close relative of S. cerevisiae). A comparison of the S. douglasii and S. cerevisiae sequences shows that there are 14% nucleotide substitutions in the coding region, with transitions being three times more frequent than transversions. At the protein level sequence identity is 87%. We have demonstrated that the S. douglasii CBP2 gene is essential for respiratory growth in the presence of a wild-type S. douglasii mitochondrial genome, but not in the presence of an intronless S. cerevisiae mitochondrial genome. Also the S. douglasii and S. cerevisiae CBP2 genes are completely interchangeable, even though the intron bI5 is absent from the S. douglasii mitochondrial genome.
Source Title: Molecular and General Genetics
URI: http://scholarbank.nus.edu.sg/handle/10635/134126
ISSN: 00268925
Appears in Collections:Staff Publications

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