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|Title:||The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induces changes in the heme spin state of microsomal cytochrome P-450|
|Authors:||Shahi, G.S. |
|Source:||Shahi, G.S., Moochhala, S.M., Das, N.P., Sunamoto, J., Tajima, K. (1990). The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induces changes in the heme spin state of microsomal cytochrome P-450. Biochemistry International 22 (5) : 895-902. ScholarBank@NUS Repository.|
|Abstract:||In vitro studies on the nature of interaction of the neurotoxin MPTP with hepatic microsomal cytochrome P-450 were carried out. Spectral perturbation studies showed nitrogenous ligand type binding between MPTP and cytochrome P-450 with a peak at 423 nm and a broad trough at 400 nm. Scatchard analysis of MPTP-cytochrome P-450 binding suggested that MPTP binds to at least 2 species of cytochrome P-450 - a high affinity binding species with an apparent spectral dissociation constant (K(s)) of 372 μM and a low affinity species with K(s) of 37.6 mM. EPR studies confirmed that MPTP is a type II substrate for the forms of cytochrome P-450 with which it interacts and causes a shift from the high spin state of cytochrome P-450 to the low spin state. MPTP is, thus, likely to be an effective inhibitor of cytochrome P-450.|
|Source Title:||Biochemistry International|
|Appears in Collections:||Staff Publications|
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