Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13401
Title: High-throughput methodologies for systematic enzyme profiling
Authors: UTTAMCHANDANI MAHESH
Keywords: HIGH-THROUGHPUT SCREENING, ENZYMOLOGY, MICROARRAYS, CHEMICAL BIOLOGY, PROTEOMICS
Issue Date: 14-Nov-2007
Source: UTTAMCHANDANI MAHESH (2007-11-14). High-throughput methodologies for systematic enzyme profiling. ScholarBank@NUS Repository.
Abstract: Recent evidence suggests that 18-29% of eukaryotic genomes encode enzymes. However, little is understood about the physiological roles, substrate specificity and downstream targets of the vast majority of these important proteins. While advances in sequencing and molecular biology have made it feasible to quickly amass a wealth of genetic information, similar capabilities are severely lacking in the relatively nascent proteomics arena. My work addresses these challenges by introducing a series of technologies that span various analytical modes, effectively expanding current capabilities in protein profiling by leveraging on throughput. These include microplate and microarray platforms, for which I demonstrate with examples, novel methodologies to garner implicit functional understanding of enzymes through systematic in vitro and in silico experimentation. Cohesively, the methodologies are applied (but not limited) to investigations of metalloproteases; an important cluster of enzymes, whose expansive biological roles present pharmaceutical importance in combating diseases like cancer, arthritis and anthrax.
URI: http://scholarbank.nus.edu.sg/handle/10635/13401
Appears in Collections:Ph.D Theses (Open)

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