Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13397
Title: Role of interleukin-13 on the development of minimal change nephrotic syndrome - A novel animal model
Authors: LAI KIN WAI
Keywords: Interleukin-13, MCNS, nephrin, podocin, dystroglycan, B7-1
Issue Date: 29-Aug-2007
Source: LAI KIN WAI (2007-08-29). Role of interleukin-13 on the development of minimal change nephrotic syndrome - A novel animal model. ScholarBank@NUS Repository.
Abstract: IL-13 has been implicated in the pathogenesis of minimal change nephrotic syndrome (MCNS). This study aimed to investigate the role of IL-13 on the development of proteinuria and expression of podocyte-related genes associated with nephrotic syndrome. IL-13 was overexpressed in Wistar rats through transfection of a mammalian expression vector cloned with the rat IL-13 gene by in-vivo electroporation. The IL-13 transfected rats (n=41) showed significant proteinuria, hypoalbuminemia and hypercholesterolemia when compared to control rats (n=17). No significant histological changes were observed in the glomeruli of IL-13- transfected rats except effacement of podocyte foot processes. Glomerular gene and protein expression was significantly upregulated for B7-1 and IL-4RI?, but downregulated for nephrin, podocin and dystroglycan in IL-13-transfected rats compared to controls. In conclusion, overexpression of IL-13 causes podocyte injury and induces minimal change-like nephropathy in rat.
URI: http://scholarbank.nus.edu.sg/handle/10635/13397
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Lai Kin Wai PhD Thesis (Final version).pdf4.69 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

310
checked on Dec 11, 2017

Download(s)

582
checked on Dec 11, 2017

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.