Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13372
Title: Hydrogen peroxide as a potential biomarker of oxidative stress: is there a reliable assay?
Authors: MOHAMED SAH REDHA BIN HAMZAH
Keywords: urinary hydrogen peroxide, biomarker, DCFH assay
Issue Date: 31-Oct-2007
Source: MOHAMED SAH REDHA BIN HAMZAH (2007-10-31). Hydrogen peroxide as a potential biomarker of oxidative stress: is there a reliable assay?. ScholarBank@NUS Repository.
Abstract: Oxidative stress in humans contributes to the development of several diseases such as cancer, diabetes, cardiovascular and neurodegenerative disorders. Hydrogen peroxide (H2O2) can be easily and cheaply detected in human urine. In order to investigate the suitability of urinary H2O2 as a biomarker of oxidative stress, a highly specific, sensitive and reproducible assay is required. Current methods of H2O2 measurement in urine samples (by FOX-2 and O2 electrode assays) were examined, and various other peroxidase-based and non-enzymatic, chemical-based methods were developed and tested. The classical oxygen electrode assay and the newly-developed, DCFH peroxidase-based assay were the two most reliable assays. The latter could detect a basal level of urinary H2O2 excreted by healthy individuals, with little intra-individual variation throughout the day and between different days. In future, urinary H2O2 can be further studied with the DCFH assay, in known pathological conditions and to see the effect of intervention with antioxidant therapy.
URI: http://scholarbank.nus.edu.sg/handle/10635/13372
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