Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/13353
Title: Chemical drug-assisted gene transfer: A sensible approach to improve transgene expression in the central nervous system
Authors: GUO HAIYAN
Keywords: gene delivery, central nervous system, polyethylenimine,P-glycoprotein, baculovirus
Issue Date: 9-Jul-2007
Source: GUO HAIYAN (2007-07-09). Chemical drug-assisted gene transfer: A sensible approach to improve transgene expression in the central nervous system. ScholarBank@NUS Repository.
Abstract: Safe and efficient gene delivery vectors are in great demand in the field of gene transfer into cells in the central nervous system (CNS). In this study, we have explored the possibility of using currently available chemical drugs or molecules to improve the gene transfer efficiency of non-viral and viral vectors. Through the modification of low molecular weight polyethylenimine (PEI) with the commonly used drug carrier i??-Cyclodextrin (CyD), a novel non-viral vector was generated, which greatly enhanced gene transfection efficiency over low MW PEI. In addition, a new approach was developed to enhance PEI-mediated gene transfection, in which the function of P-glycoprotein efflux pump, well known as a main reason for tumor cell chemotherapy resistance, was inhibited using either pharmaceutical or molecular biology approaches. Furthermore, we worked on novel baculovirus vectors and found enhanced therapeutic effects in gliomas by combining baculovirus with sodium butyrate, a histone deacetylase inhibitor.
URI: http://scholarbank.nus.edu.sg/handle/10635/13353
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