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Title: | Chemical drug-assisted gene transfer: A sensible approach to improve transgene expression in the central nervous system | Authors: | GUO HAIYAN | Keywords: | gene delivery, central nervous system, polyethylenimine,P-glycoprotein, baculovirus | Issue Date: | 9-Jul-2007 | Citation: | GUO HAIYAN (2007-07-09). Chemical drug-assisted gene transfer: A sensible approach to improve transgene expression in the central nervous system. ScholarBank@NUS Repository. | Abstract: | Safe and efficient gene delivery vectors are in great demand in the field of gene transfer into cells in the central nervous system (CNS). In this study, we have explored the possibility of using currently available chemical drugs or molecules to improve the gene transfer efficiency of non-viral and viral vectors. Through the modification of low molecular weight polyethylenimine (PEI) with the commonly used drug carrier i??-Cyclodextrin (CyD), a novel non-viral vector was generated, which greatly enhanced gene transfection efficiency over low MW PEI. In addition, a new approach was developed to enhance PEI-mediated gene transfection, in which the function of P-glycoprotein efflux pump, well known as a main reason for tumor cell chemotherapy resistance, was inhibited using either pharmaceutical or molecular biology approaches. Furthermore, we worked on novel baculovirus vectors and found enhanced therapeutic effects in gliomas by combining baculovirus with sodium butyrate, a histone deacetylase inhibitor. | URI: | http://scholarbank.nus.edu.sg/handle/10635/13353 |
Appears in Collections: | Ph.D Theses (Open) |
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