Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/133358
Title: Identification of CD8+ T-cell epitopes specific for immediate-early transactivator Rta of Epstein-Barr virus
Authors: Yu, H.
Srinivasan, N.
Ren, E. 
Chan, S. 
Keywords: EBV
Epitopes
HLA
T lymphocytes
Tumor immunology
Issue Date: May-2005
Citation: Yu, H., Srinivasan, N., Ren, E., Chan, S. (2005-05). Identification of CD8+ T-cell epitopes specific for immediate-early transactivator Rta of Epstein-Barr virus. Human Immunology 66 (5) : 483-493. ScholarBank@NUS Repository.
Abstract: Nasopharyngeal carcinoma (NPC) is a human epithelial tumor with a high incidence in Southern Chinese population, with contributions from Epstein-Barr virus (EBV), human leukocyte antigen (HLA), and environmental factors to its etiology. It has been shown previously that the recognition of immediate-early transactivator Rta of EBV by CD8+ T cells may have a significant impact on controlling EBV and, indirectly, NPC. The current study used two computer-aided prediction methods and competition-based HLA-peptide binding assays to screen for HLA B2704/B4601/B5801 restricted T-cell epitopes derived from Rta. HLA tetrameric complexes containing these potential T-cell epitopes were synthesized. Rta-specific CD8+ T-cell responses in healthy virus carriers were then defined by these tetramers and IFN-γ ELISPOT assays. We clearly demonstrated that healthy virus carriers have detectable Rta-specific CD8+ T cells restricted by B2704 in the circulation. However, there were no B4601/B5801 tetramer-reactive T cells specific for Rta in the peripheral blood of matched/mismatched donors. On the other hand, B4601 tetramers containing the computer-predicted B4601 binder EBNA3A (318-326) showed detectable tetramer-reactive T cells in the circulation of healthy virus carriers. topes also elicited IFN-γ responses as detected by ELISPOT. © American Society for Histocompatibility and Immunogenetics, 2005. Published by Elsevier Inc.
Source Title: Human Immunology
URI: http://scholarbank.nus.edu.sg/handle/10635/133358
ISSN: 01988859
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

3
checked on Mar 7, 2018

WEB OF SCIENCETM
Citations

3
checked on May 7, 2018

Page view(s)

20
checked on Feb 25, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.