Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/133137
Title: GEOMETRIC CONTROL OF 3D CHROMOSOME ORGANIZATIONS
Authors: WANG YEJUN
Keywords: Cell geometry, Chromosome position, Genome regulation, Mechanobiology, Superresolution Microscopy, Chromosomal contacts
Issue Date: 29-Jul-2016
Source: WANG YEJUN (2016-07-29). GEOMETRIC CONTROL OF 3D CHROMOSOME ORGANIZATIONS. ScholarBank@NUS Repository.
Abstract: Extracellular matrix (ECM) signals regulate gene expression program, which is central to tissue and cell homeostasis such as cell proliferation, apoptosis, differentiation, as well as tumor initiation and progression. Regulation of eukaryotic genes occurs through various layers including 3D chromosome positioning, chromatin remodeling, and chromosome physical interactions. While such ECM- regulated modular gene expression is remarkable, how these mechanical signals are integrated into the 3D chromosome architecture is not clear. In the first project, we quantitatively investigated the role of cell geometry on 3D chromosome position and revealed its implications in genome regulation. In the second project, we developed open chromatin spreads to visualize decondensed chromatin structures and nanoscale chromosomal contacts at active transcription sites using superresolution microscopy. With this system we discovered that cell geometry regulates the cellular level of chromosomal contacts associated with specific transcription factors, while differentially regulating their target genes. Taken together, this work provides a quantitative framework together with a robust open chromatin platform to systematically understand the role of cell geometry in 3D chromosome reorganization for genome regulation.
URI: http://scholarbank.nus.edu.sg/handle/10635/133137
Appears in Collections:Ph.D Theses (Open)

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