Please use this identifier to cite or link to this item: https://doi.org/10.1079/IVP2002340
Title: In vitro flower induction in roses
Authors: Wang, G.Y.
Yuan, M.F.
Hong, Y. 
Keywords: In vitro flowering
Phytohormones
Plant age in culture
Rose
Subculture time
Issue Date: Sep-2002
Source: Wang, G.Y., Yuan, M.F., Hong, Y. (2002-09). In vitro flower induction in roses. In Vitro Cellular and Developmental Biology - Plant 38 (5) : 513-518. ScholarBank@NUS Repository. https://doi.org/10.1079/IVP2002340
Abstract: Vegetatively propagated plantlets of six rose cultivars were induced to flower in vitro on media containing full-strength Murashige and Skoog (MS) inorganic salts, Gamborg's B5 organic elements with 400 mgl-1 myo-inositol, and different phytohormone combinations of 6-benzyladenine (BA) with α-naphthaleneacetic acid (NAA); thidiazuron (TDZ) with NAA; and zeatin (ZT) with NAA. The most efficient flower bud induction (49.1% and 44.1%) was obtained on media supplemented with 0.5 mgl-1 (2.27 μM) TDZ and 0.1 mgl-1 (0.54 μM) NAA or 0.5 mgl-1 (2.28 μM) ZT and 0.1 mgl-1 (0.54 μM) NAA for cultivar Orange Parade. Scanning electron microscopy (SEM) showed that in vitro flower bud induction occurred mostly between 15 and 30d in induction medium through the normal flower development processes. With TDZ and ZT as the best choice for flower induction in all six cultivars tested, different rose cultivars varied in their responses to phytohormone treatments. Our study also revealed that the total time from original culture and subculture time before flower induction were two very important factors for in vitro flower induction. Plantlets 156-561d from original culture and subcultured for 45d were the best for flower induction.
Source Title: In Vitro Cellular and Developmental Biology - Plant
URI: http://scholarbank.nus.edu.sg/handle/10635/132908
ISSN: 10545476
DOI: 10.1079/IVP2002340
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