Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/132813
Title: Processing scarce biological samples for light and transmission electron microscopy
Authors: Taupin, P. 
Keywords: Araldite
Bis-acrylamide
Bovine serum albumin
Cross-link
Cryostat
Embedding
LR White
Issue Date: Apr-2008
Source: Taupin, P. (2008-04). Processing scarce biological samples for light and transmission electron microscopy. European Journal of Histochemistry 52 (2) : 135-139. ScholarBank@NUS Repository.
Abstract: Light microscopy (LM) and transmission electron microscopy (TEM) aim at understanding the relationship structure-function. With advances in biology, isolation and purification of scarce populations of cells or subcellular structures may not lead to enough biological material, for processing for LM and TEM. A protocol for preparation of scarce biological samples is presented. It is based on pre-embedding the biological samples, suspensions or pellets, in bovine serum albumin (BSA) and bis-acrylamide (BA), cross-linked and polymerized. This preparation provides a simple and reproducible technique to process biological materials, present in limited quantities that can not be amplified, for light and transmission electron microscopy. ©2008 European Journal of Histochemistry.
Source Title: European Journal of Histochemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/132813
ISSN: 1121760X
Appears in Collections:Staff Publications

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