Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/132750
Title: Hatching out goldfish from common carp eggs: Interspecific androgenesis between two cyprinid species
Authors: Bercsényi, M.
Magyary, I.
Urbányi, B.
Orbán, L. 
Horváth, L.
Keywords: Nuclear-mitochondrial incompatibility
RAPD
Whole genome manipulation
Issue Date: 1998
Source: Bercsényi, M., Magyary, I., Urbányi, B., Orbán, L., Horváth, L. (1998). Hatching out goldfish from common carp eggs: Interspecific androgenesis between two cyprinid species. Genome 41 (4) : 573-579. ScholarBank@NUS Repository.
Abstract: We have successfully performed interspecific androgenesis between two cyprinid species. Gamma-ray irradiated eggs of common carp were fertilized with fresh and cryopreserved sperm of three different goldfish varieties and the haploid embryos were then heat-shocked to restore diploidy and to produce viable offspring. Androgenic diploid goldfish progenies from over a dozen different experiments were screened for four phenotypic markers several times. Color and other phenotypic markers characteristic of goldfish were found exclusively among androgenetic goldfish progenies; no markers originating from common carp were detected in over 1503 individuals investigated visually. RAPD assay was used to compare the parents and the offspring at the genomic level. The RAPD pattern of the androgenetic goldfish contained exclusively paternal bands, thereby confirming the results of the phenotypic analysis. According to our knowledge, this is the first successful interspecific androgenesis performed with two different species resulting in viable offspring.
Source Title: Genome
URI: http://scholarbank.nus.edu.sg/handle/10635/132750
ISSN: 08312796
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

6
checked on Jan 15, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.