Please use this identifier to cite or link to this item: https://doi.org/10.1007/s004380050026
Title: Molecular cloning and characterisation of a maize cDNA for a homologue of the large subunit of the eukaryotic initiation factor 3 (eIF3)
Authors: Sabelli, P.A. 
Burgess, S.R.
Valášek, L.
Shewry, P.R.
Keywords: eIF3
Maize
Protein synthesis
Translation
Issue Date: 1999
Source: Sabelli, P.A., Burgess, S.R., Valášek, L., Shewry, P.R. (1999). Molecular cloning and characterisation of a maize cDNA for a homologue of the large subunit of the eukaryotic initiation factor 3 (eIF3). Molecular and General Genetics 261 (4-5) : 820-830. ScholarBank@NUS Repository. https://doi.org/10.1007/s004380050026
Abstract: In order to identify genes that are specifically expressed in distinct cell populations of the maize root apex, we have constructed PCR-directed cDNA libraries from microdissected populations of cells, and screened them by differential hybridisation. A meristem-specific cDNA was isolated and characterised. This cDNA, termed ZmeIF3A, encodes a protein homologous to the large subunit of the eukaryotic translation Initiation Factor 3 (eIF3), which is an essential multi-protein complex for the initiation of protein synthesis. The ZmeIF3A protein is most similar to the yeast homologue RPG1, lacking the repeated C-terminal domain characteristic of its mammalian counterparts. However, despite this similarity, it fails to replace the RPG1 protein in complementation experiments on yeast mutants. Analysis of gene expression in situ showed that the ZmeIFsA transcript is expressed in the region of the root meristem surrounding the central stele. ZmeIF3A mRNA is also expressed in the young root, the male inflorescence, and the developing cob and seed. In maize, ZmeIF3A is encoded by one or two genomic sequences. This is the first report on the isolation and characterisation of a cDNA from higher plants that encodes a product homologous to a component of the eIF3 complex.
Source Title: Molecular and General Genetics
URI: http://scholarbank.nus.edu.sg/handle/10635/132744
ISSN: 00268925
DOI: 10.1007/s004380050026
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

3
checked on Jan 17, 2018

Page view(s)

11
checked on Jan 15, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.