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Title: In vitro developmental expression of dystroglycan and laminin-α2 in human skeletal muscle
Authors: Chen, L. 
Burgunder, J.-M.
Keywords: α- and β-dystroglycan
Human skeletal muscle
Post-translational modification
Issue Date: Jul-2005
Source: Chen, L., Burgunder, J.-M. (2005-07). In vitro developmental expression of dystroglycan and laminin-α2 in human skeletal muscle. Cell Biology International 29 (7) : 506-513. ScholarBank@NUS Repository.
Abstract: The α-subunit of dystroglycan, a member of the dystrophin associated protein complex, binds to extracellular laminin-α2, while its β-subunit interacts with cytoskeletal dystrophin. The exact biological role of dystroglycan, especially during human skeletal muscle development, has not been fully explored. Here, we analysed the distribution and expression characteristics of both dystroglycan subunits and laminin-α2 in primary human skeletal muscle cells. During development, expression levels of all three proteins increased with differentiation. The proteins were relocated from the sarcoplasm to the sarcolemma. The size of α-dystroglycan decreased from 150-220 kDa at the proliferation stage to 100-120 kDa at the late developmental stage. Both α- and β-dystroglycan were involved in forming a complex with their respective partners laminin-α2 and dystrophin/utrophin. Our data show that, during development, cells may employ tightly regulated post-translational species-specific modification to produce different isoforms of α-dystroglycan to participate in appropriate functions. © 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
Source Title: Cell Biology International
ISSN: 10656995
DOI: 10.1016/j.cellbi.2005.01.009
Appears in Collections:Staff Publications

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