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|Title:||The blood-testis barrier in the rabbit - A light microscopic study using horseradish peroxidase (HRP)|
|Authors:||Singh, G. |
|Source:||Singh, G., Yang, W.M. (1982). The blood-testis barrier in the rabbit - A light microscopic study using horseradish peroxidase (HRP). IRCS Medical Science 10 (8) : 614-615. ScholarBank@NUS Repository.|
|Abstract:||The blood-testis barrier was studied in rabbits using horseradish peroxidase (HRP - Sigma Type VI) as a biological tracer molecule. It was injected directly into the testicular artery, and at intervals of 5 and 30 minutes, a sample of the testis was obtained and processed for the HRP reaction using tetramethyl benzidine (TMB) as a chromogen. Contrary to the classical view that the blood-testis barrier is synonymous with the Sertoli cell barrier, it was found that the basement membrane of the seminiferous tubule is a structure of some importance in contributing to the barrier state. No HRP activity was demonstrable within the lumen of the seminiferous tubule. Maximum sites of HRP activity were seen in the interstitial spaces surrounding the tubule. It is concluded that the blood-testis barrier needs to be looked at further before incriminating the occluding junctions at the bases of Sertoli cells as the site of the barrier state.|
|Source Title:||IRCS Medical Science|
|Appears in Collections:||Staff Publications|
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