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|Title:||The c-erbAβ thyroid hormone receptor: Expression and cDNA sequence analysis of the hormone-binding domain in human cancer cell lines|
|Authors:||Chow, V.T.K. |
|Citation:||Chow, V.T.K., Lim, S.S., Tock, E.P.C. (1994). The c-erbAβ thyroid hormone receptor: Expression and cDNA sequence analysis of the hormone-binding domain in human cancer cell lines. Acta Oncologica 33 (5) : 499-505. ScholarBank@NUS Repository.|
|Abstract:||The human c-erbAβ protooncogene encodes a thyroid hormone receptor (comprising a hormone-binding domain and a DNA-binding domain) which modulates expression of specific genes, such as cell differentiation genes. Using the reverse transcription and polymerase chain reaction (RT-PCR) assay, significant expression of the c-eubAβ gene was detected in the SiHa, CaSki, HeLa cervical carcinoma; Hep3B, PLC/PRF/5, Mahlavu hepatocellular carcinoma; HT-1080 fibrosarcoma cell lines; as well as in normal MRC-5 embryo lung and FS-4 foreskin fibroblast cell lines. However, the Molt-4 leukaemia and Raji Burkitt's lymphoma cell lines exhibited very low levels of c-ePbAβ expression. Single-strand conformation polymorphism analysis and direct sequencing of PCR products of the c-erbAβ hormonebinding domain cDNAs of these cell lines revealed identical sequences, but differed from the published human placental c-erbAβ sequence by five single base disparities. Sequencing of an aberrant fragment fortuitously amplified from the HT-1080 cDNA library demonstrated concordance with the cDNA of pregnancy-specific glycoprotein 4, which is related to the tumour marker, carcinoembryonic antigen.|
|Source Title:||Acta Oncologica|
|Appears in Collections:||Staff Publications|
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