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|Title:||Serum IgG response to differentiated antigens of Helicobacter pylori|
|Authors:||Hua, J.-S. |
Helicobacter pylori infection
|Citation:||Hua, J.-S., Khin, M.M., Zheng, P.-Y., Yeoh, K.-G., Ng, H.-C., Ho, B. (1998). Serum IgG response to differentiated antigens of Helicobacter pylori. World Journal of Gastroenterology 4 (1-6) : 249-251. ScholarBank@NUS Repository.|
|Abstract:||AIM: To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS: Blood samples were collected from 278 patients with gastric diseases. A 3-day-old culture of H. pylori on chocolate blood agar was used to provide spiral form. 'Synchronous' coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0.4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme-linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori. RESULTS: Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non-ulcer dyspepsia (NUD) (44%) and other diseases (45%) (P|
|Source Title:||World Journal of Gastroenterology|
|Appears in Collections:||Staff Publications|
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