Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/132032
Title: Serum IgG response to differentiated antigens of Helicobacter pylori
Authors: Hua, J.-S. 
Khin, M.M. 
Zheng, P.-Y.
Yeoh, K.-G.
Ng, H.-C.
Ho, B. 
Keywords: Antigen-antibody reactions
Antigens, bacterial
Antigens, differentiation
Coccoid form
ELISA
Gastritis
Helicobacter pylori
Helicobacter pylori infection
IgG
Spiral form
Stomach neoplasms
Stomach ulcer
Issue Date: 1998
Source: Hua, J.-S., Khin, M.M., Zheng, P.-Y., Yeoh, K.-G., Ng, H.-C., Ho, B. (1998). Serum IgG response to differentiated antigens of Helicobacter pylori. World Journal of Gastroenterology 4 (1-6) : 249-251. ScholarBank@NUS Repository.
Abstract: AIM: To detect antibodies against Helicobacter pylori spiral and coccoid antigens in human sera. METHODS: Blood samples were collected from 278 patients with gastric diseases. A 3-day-old culture of H. pylori on chocolate blood agar was used to provide spiral form. 'Synchronous' coccoids were cultured in (BHY) (brain heart infusion supplemented with 10% horse serum and 0.4% yeast extract) medium in a chemostat. Antigens from spiral and coccoid form were prepared using acid glycine extraction. Enzyme-linked immunosorbent assay (ELISA) was performed to detect serum IgG antibodies against spiral and coccoid forms of H. pylori. RESULTS: Seroprevalence of H. pylori infection was higher in patients with gastric ulcer (79%) and gastric cancer (83%) than those with non-ulcer dyspepsia (NUD) (44%) and other diseases (45%) (P
Source Title: World Journal of Gastroenterology
URI: http://scholarbank.nus.edu.sg/handle/10635/132032
ISSN: 10079327
Appears in Collections:Staff Publications

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