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|Title:||T-cell receptor gene expression in tumour-infiltrating lymphocytes and peripheral blood lymphocytes of patients with nasopharyngeal carcinoma|
|Citation:||Chen, Y., Chew, C.T., Chan, S.H. (1995). T-cell receptor gene expression in tumour-infiltrating lymphocytes and peripheral blood lymphocytes of patients with nasopharyngeal carcinoma. British Journal of Cancer 72 (1) : 117-122. ScholarBank@NUS Repository.|
|Abstract:||The T-cell receptor (TCR) repertoire expression of tumour-infiltrating lymphocytes (TILs) from 19 nasopharyngeal carcinoma (NPC) biopsies was compared with those of lymphocytes from 18 control nasopharyngeal biopsies. mRNA was extracted from these lymphocytes and the cDNA transcribed. A panel of 18 Vα- and 21 Vβ-specific primers was used to detect the TCR gene use from cDNA. The use of Vα and Vβ genes was restricted in TILs compared with lymphocytes from biopsies. The frequencies of Vα2, Vα3, Vα9, Vα10, Vα11, Vα13, Vα14, Vα15, Vβ11, Vβ14, Vβ15 and Vβ20 were decreased and the frequencies of Vα10 [P(c) = 0.04; relative risk (RR) = 0.05], Vα11 (P(c) = 0.02; RR = 0.07), Vα13 (P(c) = 0.002; RR = 0), Vα14 (P(c) = 0.04; RR = 0.05), Vβ14 (P(c) = 0.001; RR = 0.03) and Vβ20 (P(c) = 0.001; RR = 0.03) remained significantly reduced after correction for the number of families typed. The frequency of Vα17 was higher in NPC biopsies than in NPC PBLs (P = 0.05), and the frequency of Vβ15 was lower in NPC biopsies than in NPC PBLs (P = 0.02). The frequencies of Vα17 and Vα18 in HLA-B46+ patients were significantly lower (P = 0.009; P = 0.044) than in B46+ controls. The results suggest that the restriction of TCR gene use in NPC patients may be important in NPC pathogenesis.|
|Source Title:||British Journal of Cancer|
|Appears in Collections:||Staff Publications|
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