Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/132003
Title: Ultrastructural changes during in vitro encystment of Blastocystis hominis
Authors: Suresh, K.
Howe, J.
Chong, S.Y.
Ng, G.C.
Ho, L.C.
Loh, A.K.
Ramachandran, N.P.
Yap, E.H. 
Singh, M.
Issue Date: 1994
Source: Suresh, K., Howe, J., Chong, S.Y., Ng, G.C., Ho, L.C., Loh, A.K., Ramachandran, N.P., Yap, E.H., Singh, M. (1994). Ultrastructural changes during in vitro encystment of Blastocystis hominis. Parasitology Research 80 (4) : 327-335. ScholarBank@NUS Repository.
Abstract: The morphological changes occurring in Blastocystis hominis at different time points following in vitro encystment were studied by electron microscopy. The following stages of the parasite were sequentially seen: (a) the amoebic form, which was irregular in shape, with a majority of the organelles being concentrated at the condensed cytoplasmic region; (b) the pre-cystic form, which was rounded and had an electron-dense material forming a homogeneous wall around the central body; and (c) the cystic form, which had a very prominent, thick osmiophilic electron-dense wall, within which there were many inclusions and possibly reproductive granules. The amoebic form appeared to be an intermediate stage between the vacuolar form and the pre-cystic form, as this stage allowed the parasite to ingest bacteria to enhance encystment. The pre-cystic stage had previously been shown in experimental infection to be infective. The role of the cystic stage in producing infection is currently being investigated.
Source Title: Parasitology Research
URI: http://scholarbank.nus.edu.sg/handle/10635/132003
ISSN: 00443255
Appears in Collections:Staff Publications

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