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|Title:||Sulphate induces very fast cell rounding and detachment|
|Authors:||Sit, K.H. |
sodium ion/proton antiport
|Citation:||Sit, K.H., Wong, K.P. (1991). Sulphate induces very fast cell rounding and detachment. Biochimica et Biophysica Acta - Molecular Cell Research 1092 (2) : 180-183. ScholarBank@NUS Repository.|
|Abstract:||Within a few minutes of incubation with SO4 2-, cultured monolayer cells retract into round shapes with drastically reduced surface area. Concomitant elevation of phosphoinositide second messenger levels, viz, 1,2-diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), is observed. A causal relationship with sulphation seems to be suggested by finding (a) sulphation of an added acceptor, 4-methylumbelliferone, (b) sulphation of endogenous glycosaminoglycan (GAG) polymers, (c) inhibition by phenol sulphotransferase inhibitor, DCNP (2,6-dichloro-4-nitrophenol). DCNP also inhibits the second messenger production and cell rounding. Reduced surface area appears to be caused by massive plasma membrane internalization in a distinctive endocytosis which is also seen in cell rounding from directly imposed ionic gradients. Reducing the surface area would reduce the adhesive or attachment sites. Besides demonstrating a highly efficient cell detachment potential, huge macromolecules appear to be readily internalized. The association of sulphation, signal transduction and cell detachment is novel.|
|Source Title:||Biochimica et Biophysica Acta - Molecular Cell Research|
|Appears in Collections:||Staff Publications|
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