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https://scholarbank.nus.edu.sg/handle/10635/13181
DC Field | Value | |
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dc.title | Role and regulation of mitochondrial permeability transition in cell death | |
dc.contributor.author | ZHANG DAWEI | |
dc.date.accessioned | 2010-04-08T10:30:43Z | |
dc.date.available | 2010-04-08T10:30:43Z | |
dc.date.issued | 2008-03-19 | |
dc.identifier.citation | ZHANG DAWEI (2008-03-19). Role and regulation of mitochondrial permeability transition in cell death. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/13181 | |
dc.description.abstract | The role of the Mitochondrial Permeability Transition (MPT) in apoptosis and necrosis is controversial. Here we show that the MPT regulates the release of cytochrome c for apoptosis during endoplasmic reticulum (ER) stress by remodeling the cristae junction (CJ). CEM cells were treated with the ER-stressor thapsigargin (THG) which led to cyclophilin-D-dependent mitochondrial release of the profusion GTPase Optic Atrophy 1 (OPA1), which controls CJ integrity, and cytochrome c leading to apoptosis. Interference RNA knockdown of Bax blocked OPA1 and cytochrome c release after THG treatment, but did not prevent the MPT showing that Bax was essential for cytochrome c release by MPT. In isolated mitochondria, MPT led to OPA1 and cytochrome c release independently of VDAC and the outer membrane indicating that the MPT is an inner membrane phenomenon. Lastly, the MPT was regulated by the electron transport chain since THG-induced cell death was inhibited in cells lacking mtDNA. | |
dc.language.iso | en | |
dc.subject | mitochondrial permeability transition, cytochrome c, OPA1, cyclophilin-D, cristae remodeling, apoptosis | |
dc.type | Thesis | |
dc.contributor.department | BIOCHEMISTRY | |
dc.contributor.supervisor | JEFFREY S ARMSTRONG | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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Zhang Dawei PhD thesis (2007 Biochemistry NUS).pdf | 16.74 MB | Adobe PDF | OPEN | None | View/Download |
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