Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/131761
Title: Molecular typing of Neisseria gonorrhoeae
Authors: Poh, C.L. 
Keywords: Gonococcus
Gonorrhoea
Molecular typing
Neisseria gonorrhoeae
Issue Date: 1998
Source: Poh, C.L. (1998). Molecular typing of Neisseria gonorrhoeae. Reviews in Medical Microbiology 9 (1) : 1-8. ScholarBank@NUS Repository.
Abstract: Phenotypic characterization of Neisseria gonorrhoeae by auxotype-serovar (A/S) classification is currently the most widely established method for differentiation of gonococci. However, the A/S classification system has some major limitations such as non-repoducibility of serotyping, especially when different batches of monoclonal antibody typing reagents are used, and the inability to differentiate strains present within major serovars. To overcome some of the limitations of the A/S classification system, newer molecular typing approaches such as restriction endonuclease analysis (REA), pulsed-field gel electrophoresis (PFGE) based on the contour-clamped, homogenous electric field electrophoresis (CHEF) technique, restriction fragment length polymorphisms of rRNA genes, multilocus enzyme electrophoretic analysis, opa-typing, direct sequencing of PIB gene and several PCR-based forms of analysis have been developed. Most of the molecular typing methods have been shown to have greater discriminatory power than serotyping. Further evaluation and comparison of the various molecular typing methods in terms of reproducibility and discriminatory power, using larger sample size of defined clinical and epidemiological origins, are needed.
Source Title: Reviews in Medical Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/131761
ISSN: 0954139X
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

6
checked on Jan 14, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.